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it binds with Vasn bound to precoated antibody
only those wells that contain Human APMAP
HACE1 present in the sample is added and binds to antibodies coated on the wells
Calculate the concentration of Anxa5 in the sample by plotting standard curve
Then a biotinylated detection antibody specific for Mouse SP-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rat SDF-1Alpha (Stromal Cell Derived Factor 1Alpha) ELISA Kit Packaging:96 wells it binds with Vasn boundThe Rat SDF 1Alpha (Stromal Cell Derived Factor 1Alpha) ELISA Kit measures SDF 1Alpha in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SDF 1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
